quality control strain s aureus atcc 25923 Search Results


strain atcc 25923  (ATCC)
99
ATCC strain atcc 25923
Example showing the real-time detection on a Smart Cycler of a fluoroquinolone-sensitive S. aureus strain (ATCC 25923) containing no mutation in the grlA gene (A) and a fluoroquinolone-resistant S. aureus strain (CCRI-12876) containing mutations at both codons 80 and 84 (B). For every sample tested, two separate PCRs, one containing probes S80-FAM and Sau-TET and one containing probes E84-FAM and Sau-TET, were performed. The left graphs show the fluorescence curves for the Sau-TET probe (hatched curve) and the S80-FAM probe (plain curve). The right graphs show the fluorescence curves for the Sau-TET probe (hatched curve) and the E84-FAM probe (plain curve). PCR assays were performed with crude DNA extracts prepared from bacterial suspensions whose turbidities were adjusted to that of a 0.5 McFarland standard.
Strain Atcc 25923, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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microbial strains  (ATCC)
99
ATCC microbial strains
Example showing the real-time detection on a Smart Cycler of a fluoroquinolone-sensitive S. aureus strain (ATCC 25923) containing no mutation in the grlA gene (A) and a fluoroquinolone-resistant S. aureus strain (CCRI-12876) containing mutations at both codons 80 and 84 (B). For every sample tested, two separate PCRs, one containing probes S80-FAM and Sau-TET and one containing probes E84-FAM and Sau-TET, were performed. The left graphs show the fluorescence curves for the Sau-TET probe (hatched curve) and the S80-FAM probe (plain curve). The right graphs show the fluorescence curves for the Sau-TET probe (hatched curve) and the E84-FAM probe (plain curve). PCR assays were performed with crude DNA extracts prepared from bacterial suspensions whose turbidities were adjusted to that of a 0.5 McFarland standard.
Microbial Strains, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bacterial strains  (ATCC)
98
ATCC bacterial strains
Example showing the real-time detection on a Smart Cycler of a fluoroquinolone-sensitive S. aureus strain (ATCC 25923) containing no mutation in the grlA gene (A) and a fluoroquinolone-resistant S. aureus strain (CCRI-12876) containing mutations at both codons 80 and 84 (B). For every sample tested, two separate PCRs, one containing probes S80-FAM and Sau-TET and one containing probes E84-FAM and Sau-TET, were performed. The left graphs show the fluorescence curves for the Sau-TET probe (hatched curve) and the S80-FAM probe (plain curve). The right graphs show the fluorescence curves for the Sau-TET probe (hatched curve) and the E84-FAM probe (plain curve). PCR assays were performed with crude DNA extracts prepared from bacterial suspensions whose turbidities were adjusted to that of a 0.5 McFarland standard.
Bacterial Strains, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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strains staphylococcus aureus atcc 25923  (ATCC)
99
ATCC strains staphylococcus aureus atcc 25923
Example showing the real-time detection on a Smart Cycler of a fluoroquinolone-sensitive S. aureus strain (ATCC 25923) containing no mutation in the grlA gene (A) and a fluoroquinolone-resistant S. aureus strain (CCRI-12876) containing mutations at both codons 80 and 84 (B). For every sample tested, two separate PCRs, one containing probes S80-FAM and Sau-TET and one containing probes E84-FAM and Sau-TET, were performed. The left graphs show the fluorescence curves for the Sau-TET probe (hatched curve) and the S80-FAM probe (plain curve). The right graphs show the fluorescence curves for the Sau-TET probe (hatched curve) and the E84-FAM probe (plain curve). PCR assays were performed with crude DNA extracts prepared from bacterial suspensions whose turbidities were adjusted to that of a 0.5 McFarland standard.
Strains Staphylococcus Aureus Atcc 25923, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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gram positive bacteria  (ATCC)
97
ATCC gram positive bacteria
Example showing the real-time detection on a Smart Cycler of a fluoroquinolone-sensitive S. aureus strain (ATCC 25923) containing no mutation in the grlA gene (A) and a fluoroquinolone-resistant S. aureus strain (CCRI-12876) containing mutations at both codons 80 and 84 (B). For every sample tested, two separate PCRs, one containing probes S80-FAM and Sau-TET and one containing probes E84-FAM and Sau-TET, were performed. The left graphs show the fluorescence curves for the Sau-TET probe (hatched curve) and the S80-FAM probe (plain curve). The right graphs show the fluorescence curves for the Sau-TET probe (hatched curve) and the E84-FAM probe (plain curve). PCR assays were performed with crude DNA extracts prepared from bacterial suspensions whose turbidities were adjusted to that of a 0.5 McFarland standard.
Gram Positive Bacteria, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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reference strain atcc 25923  (ATCC)
99
ATCC reference strain atcc 25923
Example showing the real-time detection on a Smart Cycler of a fluoroquinolone-sensitive S. aureus strain (ATCC 25923) containing no mutation in the grlA gene (A) and a fluoroquinolone-resistant S. aureus strain (CCRI-12876) containing mutations at both codons 80 and 84 (B). For every sample tested, two separate PCRs, one containing probes S80-FAM and Sau-TET and one containing probes E84-FAM and Sau-TET, were performed. The left graphs show the fluorescence curves for the Sau-TET probe (hatched curve) and the S80-FAM probe (plain curve). The right graphs show the fluorescence curves for the Sau-TET probe (hatched curve) and the E84-FAM probe (plain curve). PCR assays were performed with crude DNA extracts prepared from bacterial suspensions whose turbidities were adjusted to that of a 0.5 McFarland standard.
Reference Strain Atcc 25923, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/quality+control+strain+s+aureus+atcc+25923/Staphylococcus+aureus%3B+subsp%2E+aureus/pm40441980-125-59-61
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strains  (ATCC)
96
ATCC strains
Example showing the real-time detection on a Smart Cycler of a fluoroquinolone-sensitive S. aureus strain (ATCC 25923) containing no mutation in the grlA gene (A) and a fluoroquinolone-resistant S. aureus strain (CCRI-12876) containing mutations at both codons 80 and 84 (B). For every sample tested, two separate PCRs, one containing probes S80-FAM and Sau-TET and one containing probes E84-FAM and Sau-TET, were performed. The left graphs show the fluorescence curves for the Sau-TET probe (hatched curve) and the S80-FAM probe (plain curve). The right graphs show the fluorescence curves for the Sau-TET probe (hatched curve) and the E84-FAM probe (plain curve). PCR assays were performed with crude DNA extracts prepared from bacterial suspensions whose turbidities were adjusted to that of a 0.5 McFarland standard.
Strains, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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s aureus atcc 25923 standard strain  (ATCC)
98
ATCC s aureus atcc 25923 standard strain
Example showing the real-time detection on a Smart Cycler of a fluoroquinolone-sensitive S. aureus strain (ATCC 25923) containing no mutation in the grlA gene (A) and a fluoroquinolone-resistant S. aureus strain (CCRI-12876) containing mutations at both codons 80 and 84 (B). For every sample tested, two separate PCRs, one containing probes S80-FAM and Sau-TET and one containing probes E84-FAM and Sau-TET, were performed. The left graphs show the fluorescence curves for the Sau-TET probe (hatched curve) and the S80-FAM probe (plain curve). The right graphs show the fluorescence curves for the Sau-TET probe (hatched curve) and the E84-FAM probe (plain curve). PCR assays were performed with crude DNA extracts prepared from bacterial suspensions whose turbidities were adjusted to that of a 0.5 McFarland standard.
S Aureus Atcc 25923 Standard Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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n 7 pseudomonas aeruginosa atcc27853 1 n 8 legionellae bacillus isolated strains  (ATCC)
99
ATCC n 7 pseudomonas aeruginosa atcc27853 1 n 8 legionellae bacillus isolated strains
Figure 2 Confirmation and verification of E. faecalis-MCDA products. (A) The E. faecalis-MCDA amplification products were detected by the MG method through visual observation of the color change. (B) LFB was applied for the visual detection of E. faecalis-MCDA products. Tube 1/Biosensor 1: positive amplification of E. faecalis strain 29212; Tube 2/Biosensor 2: negative amplification of Enterococcus faecium <t>(2nd</t> GZUTCM); Tube 3/Biosensor 3: negative amplification of Staphylococcus aureus (ATCC25923); Tube 4/Biosensor 4: blank control (DW).
N 7 Pseudomonas Aeruginosa Atcc27853 1 N 8 Legionellae Bacillus Isolated Strains, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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reference strains  (ATCC)
99
ATCC reference strains
Figure 2 Confirmation and verification of E. faecalis-MCDA products. (A) The E. faecalis-MCDA amplification products were detected by the MG method through visual observation of the color change. (B) LFB was applied for the visual detection of E. faecalis-MCDA products. Tube 1/Biosensor 1: positive amplification of E. faecalis strain 29212; Tube 2/Biosensor 2: negative amplification of Enterococcus faecium <t>(2nd</t> GZUTCM); Tube 3/Biosensor 3: negative amplification of Staphylococcus aureus (ATCC25923); Tube 4/Biosensor 4: blank control (DW).
Reference Strains, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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whole bacteria s aureus bacterial samples  (ATCC)
99
ATCC whole bacteria s aureus bacterial samples
Figure 2 Confirmation and verification of E. faecalis-MCDA products. (A) The E. faecalis-MCDA amplification products were detected by the MG method through visual observation of the color change. (B) LFB was applied for the visual detection of E. faecalis-MCDA products. Tube 1/Biosensor 1: positive amplification of E. faecalis strain 29212; Tube 2/Biosensor 2: negative amplification of Enterococcus faecium <t>(2nd</t> GZUTCM); Tube 3/Biosensor 3: negative amplification of Staphylococcus aureus (ATCC25923); Tube 4/Biosensor 4: blank control (DW).
Whole Bacteria S Aureus Bacterial Samples, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Example showing the real-time detection on a Smart Cycler of a fluoroquinolone-sensitive S. aureus strain (ATCC 25923) containing no mutation in the grlA gene (A) and a fluoroquinolone-resistant S. aureus strain (CCRI-12876) containing mutations at both codons 80 and 84 (B). For every sample tested, two separate PCRs, one containing probes S80-FAM and Sau-TET and one containing probes E84-FAM and Sau-TET, were performed. The left graphs show the fluorescence curves for the Sau-TET probe (hatched curve) and the S80-FAM probe (plain curve). The right graphs show the fluorescence curves for the Sau-TET probe (hatched curve) and the E84-FAM probe (plain curve). PCR assays were performed with crude DNA extracts prepared from bacterial suspensions whose turbidities were adjusted to that of a 0.5 McFarland standard.

Journal:

Article Title: Real-Time PCR Assay for Detection of Fluoroquinolone Resistance Associated with grlA Mutations in Staphylococcus aureus

doi: 10.1128/JCM.41.7.3246-3251.2003

Figure Lengend Snippet: Example showing the real-time detection on a Smart Cycler of a fluoroquinolone-sensitive S. aureus strain (ATCC 25923) containing no mutation in the grlA gene (A) and a fluoroquinolone-resistant S. aureus strain (CCRI-12876) containing mutations at both codons 80 and 84 (B). For every sample tested, two separate PCRs, one containing probes S80-FAM and Sau-TET and one containing probes E84-FAM and Sau-TET, were performed. The left graphs show the fluorescence curves for the Sau-TET probe (hatched curve) and the S80-FAM probe (plain curve). The right graphs show the fluorescence curves for the Sau-TET probe (hatched curve) and the E84-FAM probe (plain curve). PCR assays were performed with crude DNA extracts prepared from bacterial suspensions whose turbidities were adjusted to that of a 0.5 McFarland standard.

Article Snippet: GenBank accession numbers for the 220-bp partial sequence of the S. aureus grlA gene are as follows: {"type":"entrez-nucleotide","attrs":{"text":"AY234038","term_id":"32453419","term_text":"AY234038"}} AY234038 for strain ATCC 25923, {"type":"entrez-nucleotide","attrs":{"text":"AY214125","term_id":"32453409","term_text":"AY214125"}} AY214125 for strain ATCC 29213, {"type":"entrez-nucleotide","attrs":{"text":"AY214123","term_id":"32453405","term_text":"AY214123"}} AY214123 for strain CCRI-2024, {"type":"entrez-nucleotide","attrs":{"text":"AY214124","term_id":"32453407","term_text":"AY214124"}} AY214124 for strain CCRI-12876, {"type":"entrez-nucleotide","attrs":{"text":"AY214128","term_id":"32453415","term_text":"AY214128"}} AY214128 for strain 9015, {"type":"entrez-nucleotide","attrs":{"text":"AY214127","term_id":"32453413","term_text":"AY214127"}} AY214127 for strain 9191, {"type":"entrez-nucleotide","attrs":{"text":"AY214126","term_id":"32453411","term_text":"AY214126"}} AY214126 for strain 9283, and {"type":"entrez-nucleotide","attrs":{"text":"AY214129","term_id":"32453417","term_text":"AY214129"}} AY214129 for strain 9299.

Techniques: Mutagenesis, Fluorescence

Figure 2 Confirmation and verification of E. faecalis-MCDA products. (A) The E. faecalis-MCDA amplification products were detected by the MG method through visual observation of the color change. (B) LFB was applied for the visual detection of E. faecalis-MCDA products. Tube 1/Biosensor 1: positive amplification of E. faecalis strain 29212; Tube 2/Biosensor 2: negative amplification of Enterococcus faecium (2nd GZUTCM); Tube 3/Biosensor 3: negative amplification of Staphylococcus aureus (ATCC25923); Tube 4/Biosensor 4: blank control (DW).

Journal: Infection and Drug Resistance

Article Title:

A Novel Detection of Enterococcus faecalis Using Multiple Cross Displacement Amplification Linked with Gold Nanoparticle Lateral Flow Biosensor

doi: 10.2147/idr.s235325

Figure Lengend Snippet: Figure 2 Confirmation and verification of E. faecalis-MCDA products. (A) The E. faecalis-MCDA amplification products were detected by the MG method through visual observation of the color change. (B) LFB was applied for the visual detection of E. faecalis-MCDA products. Tube 1/Biosensor 1: positive amplification of E. faecalis strain 29212; Tube 2/Biosensor 2: negative amplification of Enterococcus faecium (2nd GZUTCM); Tube 3/Biosensor 3: negative amplification of Staphylococcus aureus (ATCC25923); Tube 4/Biosensor 4: blank control (DW).

Article Snippet: 94 .1 74 .2 8 on 1 3- D ec -2 01 9 F or p er so na l u se o nl y. Powered by TCPDF (www.tcpdf.org) 1 / 1 Table 2 Bacterial Strains Used in the Current Study No. Bacteria Strain No. (Source of Strains) No. of Strains MCDA-LFB Result 1 E. faecalis ATCC 29212 1 P 2 E. faecalis Isolated strains (2nd GZUTCM) 12 P 3 Enterococcus faecium Isolated strains (2nd GZUTCM) 9 N 4 Enterococcus avium Isolated strains (2nd GZUTCM) 1 N 5 Enterococcus raffinosus Isolated strains (2nd GZUTCM) 1 N 6 Staphylococcus aureus ATCC 25923 1 N 7 Pseudomonas aeruginosa ATCC27853 1 N 8 Legionellae bacillus Isolated strains (2nd GZUTCM) 1 N 9 Hemophililus parainfluenza Isolated strains (2th GZUTCM) 1 N 10 Bordetella parapertussis Isolated strains (2nd GZUTCM) 1 N 11 Klebsiella pneumoniae Isolated strains (2nd GZUTCM) 1 N 12 Shigella boydii Isolated strains (2nd GZUTCM) 1 N 13 Mycoplasma pneumoniae Isolated strains (2nd GZUTCM) 1 N 14 Enteropathogenic Escherichia coli Isolated strains (GZCDC) 1 N 15 Streptococcus pneumoniae Isolated strains (2nd GZUTCM) 1 N 16 Staphylococcus saprophyticus Isolated strains (GZCDC) 1 N 17 Enterotoxigenic Escherichia coli Isolated strains (2nd GZUTCM) 1 N 18 Invasive Escherichia coli Isolated strains (2nd GZUTCM) 1 N 19 Enterohemorrhagic Escherichia coli Isolated strains (2nd GZUTCM) 1 N 20 Enteroaggregative Escherichia coli Isolated strains (2nd GZUTCM) 1 N 21 Streptococcus suis Isolated strains (GZCDC) 1 N (Continued) submit your manuscript | www.dovepress.com DovePress Infection and Drug Resistance 2019:123774 In fe ct io n an d D ru g R es is ta nc e do w nl oa de d fr om h ttp s: //w w w .d ov ep re ss .c om / b y 10 9.

Techniques: Control